Development and characterization of a gelatin-based herbal hydrogel patch loaded with Jatropha podagrica leaf extract for antibacterial wound dressing.

Authors

  • Karnchiya Mekkala
  • Pornpavee Vongvichien Varee ChiangMai School
  • Pantila Saengphet Varee ChiangMai School
  • Winrada Chaisit Varee ChiangMai School
  • Nuttsinee Wongjiraheroun Varee ChiangMai School
  • Viwat Sutana Varee Chiangmai School

Keywords:

Jatropha podagrica, Saponin

Abstract

This study developed a hydrogel wound dressing infused with Jatropha podagrica leaf extract to evaluate its antibacterial potential. Saponin-rich extracts were obtained by ethanol extraction and solvent partitioning, then incorporated into gelatin hydrogels at varying concentrations (0–100%v/v). The antibacterial activity was assessed using the disk diffusion method. Results showed that the hydrogel with 80% extract achieved the greatest bacterial inhibition. These findings suggest that Jatropha podagrica extract enhances hydrogel efficacy, highlighting its potential as a natural antibacterial wound dressing.

References

Lethongkam, S., et al. (2025). Hydrogel dressing containing natural extracts inhibits dual-species biofilms of Staphylococcus aureus and Acinetobacter baumannii. Retrieved from https://www.nature.com/articles/s41598-025-18150-3

Yuansong Sun, et al. (2025). Hydrogen bond-induced tea saponin incorporation with cellulose hydrogels enables accelerated acute wound healing. Retrieved from https://www.sciencedirect.com/science/article/abs/pii/S0141813025067157

Tracey A. Taylor; Ellis H. Tobin; Chandrashekhar G. Unakal. (2025). Staphylococcus aureus Infection. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK441868/

Sharma, R., et al. (2025). Comprehensive review on antimicrobial-based hydrogel for wound healing. Retrieved from https://journals.stmjournals.com/rrjodfdp/article%3D2025/view%3D213586/?utm_source=chatgpt.com

Worawut Somphuet. (2024). Development of hydrogel eye patches from herbal extracts of “Ya Ha Rak”. Retrieved from https://ph01.tcithaijo.org/index.php/Scip sru/article/view/255887/173669

Jing Han, Qingxun Meng, Taicheng Liu, Mengru Lv, Wenxuan Su, Beibei Liu, Jiannan Wu. (2024). Immunomodulatory Antibacterial Hydrogel for Wound Infection Management. Retrieved from https://www.dovepress.com/immunomodulatory-antibacterial-hydrogel-for-wound-infection-management-peer-reviewed-fulltext-article-IJN

Pikhtirova, A., Pecka-Kiełb, E., & Zigo, F. (2023). Antimicrobial activity of saponin-containing plants: A review. Journal of Dairy, Veterinary & Animal Research.

Mary S. Mora-Ocación, Ana Cruz. Morillo-Coronado, Elsa Helena. Manjarres-Hernández. (2022). Extraction and Quantification of Saponins in Quinoa (Chenopodium quinoa Willd.) Genotypes from Colombia. Retrieved from https://doi.org/10.1155/2022/7287487

Truong Ngoc Minh, Tran Dang Xuan, Hoang-Dung Tran, Truong Mai Van, Yusuf Andriana, Tran Dang Khanh, Nguyen Van Quan and Ateeque Ahmad. (2019). Isolation and Purification of Bioactive Compounds from the Stem Bark of Jatropha podagrica. Retrieved from https://www.researchgate.net/profile/Tran-Khanh-14/publication/331477775_Isolation_and_Purification_of_Bioactive_Compounds_from_the_Stem_Bark_of_Jatropha_podagrica/links/5c7c1cec92851c6950520378/Isolation-and-Purification-of-Bioactive-Compounds-from-the-Stem-Bark-of-Jatropha-podagrica.pdf?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InB1YmxpY2F0aW9uIiwicGFnZSI6InB1YmxpY2F0aW9uRG93bmxvYWQiLCJwcmV2aW91c1BhZ2UiOiJwdWJsaWNhdGlvbiJ9fQ

Opeyemi Saheed Kolawole, et al. (2017). Taxonomic value of the leaf micro morphology and quantitative phytochemistry of Jatropha intergerrima Jacq. and Jatropha podagrica Hook. (Euphor-biaceae) — well-known horticultural plants in Nigeria. Retrieved from https://revistas.um.es/analesbio/article/download/420911/280951/1 417691

Apiradee Korpphaiboon et al. (2015). Development of extraction methods and evaluation of saponin efficiency from rambutan peel. Retrieved from https://www.doa.go.th/research/attachment.php?aid=1566

Duangkamol Ruen-ngam. (2014). Extraction of antioxidants. Bangkok: King Mongkut’s Institute of Technology Ladkrabang. Retrieved from https://www.thaiscience.info/journals/Article/JOSL/10970881.pdf

Duangporn Premjit, Ph.D., Associate Professor, and colleagues. (2014). Research and development project on plants in the genus Jatropha sp. for industrial-scale production. Phitsanulok: Naresuan University.

C.-J. Chen and Y.-C. Huang. (2014). New epidemiology of Staphylococcus aureus infection in Asia. Retrieved from https://www.sciencedirect.com/science/article/pii/S1198743X14611460

Runner R.T. Majinda. (2012). Extraction and Isolation of Saponins. Retrieved from https://www.researchgate.net/publication/221864189_Extraction_and_Isolation_of_Saponins

Nwokocha., Blessing, A., Agbagwa, I.O., Okoli. (2011). B.E. Comparative phytochemical screening of Jatropha L. Species in the Niger Delta. research journal of phytochemistry, vol. 5, no. 2, p. 107–114. Retrieved from https://doi.org/10.3923/rjphyto.2011.107114

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Published

2026-05-04

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