Nigella sativa derived Nano Bioactive Antimicrobial as a Next Generation Intracanal Medicament for Resistant Endodontic Pathogens
Keywords:
Nigella sativa, Enterococcus faecalis, thymoquinone, antibacterial activityAbstract
This study investigated the antimicrobial efficacy of thymoquinone, the active component of Nigella sativa, against Enterococcus faecalis (ATCC 29212) and compared it with calcium hydroxide. Thymoquinone dissolved in 10% DMSO was evaluated using MIC, MBC, and anti-adherence assays. It showed strong activity, with an MIC of 1.6 µg/ml and an MBC of 3.2 µg/ml. Sub-MIC concentrations also reduced bacterial adherence. These findings suggest thymoquinone is a promising alternative or adjunctive intracanal medicament to calcium hydroxide, supporting further investigation through clinical studies.
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